Equipment
Main ContentBD Biosciences FACSymphony A3 Cell Analyzer
The BD FACSymphony™A3 Cell Analyzer is a novel high parameter system that offers qualitative and quantitative analysis of cells and other particles using multi-parametric analysis. This instrument is configured for five laser (UV 355 nm, Violet 405 nm, Blue 488 nm, Yellow-Green 561 nm, Red 637 nm) analysis and can simultaneously acquire and distinguish between 23 different fluorescent signals in addition to the forward and side scatter parameters allowing for a 25-parameter definition of the cell. The FACSymphony has the capacity to acquire up to 40,000 events per second allowing for a rapid acquisition of samples.Additionally, the BD High-Throughput Sampler (HTS) option can be used to acquire samples from a 96 or 384-well plate. The FACSymphony is operated using FACSDiva 9.1v software. FCS file versions 2.0, 3.0, and 3.1 can be exported for data analysis.
BD FACS Symphony A3 Configuration and Fluorochromes |
Excitation Laser | Longpass Filter | Bandpass Filter | Examples of Fluorochromes | Detector |
Blue (488 nm) | 505LP | 515/20 | BB515, FITC, AF 488, CFSE, GFP, TO-PRO-1 | BB515 |
600LP | 610/20 | BB630 | BB630 |
690LP | 710/50 | BB700, PerCP, PerCP-Cy5.5, PerCP-eFluor710, 7-AAD, PI | BB700 |
770LP | 780/60 | BB790 | BB790 |
Red (637 nm) | 650LP | 670/30 | APC, AF633, AF647, TO-PRO-3 | APC |
690LP | 710/50 | AF700, APC-Cy5.5, Vybrant® DyeCycle™ Ruby Stain | AF700 |
750LP | 780/60 | APC-CY7, APC-H7, APC-eFluor780 | APC Cy7 |
Yellow-green (561 nm) | 570LP | 586/15 | PE, AF555, AF546, dTomato, DsRed, RFP | PE |
600LP | 610/20 | PI, PE/Dazzle 594, PE-CF594, PE-Texas Red, mCherry | PE CF594 |
650LP | 670/30 | PE-Cy5, 7AAD, mPlum | PE Cy5 |
750LP | 780/60 | PE-Cy7 | PE Cy7 |
Violet (405 nm) | 410LP | 431/28 | BV421 | BV421 |
410LP | 450/50 | BV421, DAPI, V450, Pacific Blue | Optional |
450LP | 470/15 | BV480 | BV480 |
600LP | 610/20 | BV605, Pacific Orange, eFluor605NC | BV605 |
635LP | 670/30 | BV650 | BV |
690LP | 710/50 | BV711, Yellow Dead Cell | BV711 |
750LP | 780/60 | BV786 | BV786 |
UV (355 nm) | none | 379/28 | BUV396 | BUV396 |
410LP | 450/50 | DAPI | DAPI |
450LP | 515/30 | BUV496 | BUV496 |
550LP | 586/15 | BUV563 | BUV563 |
635LP | 670/30 | BUV661 | BUV661 |
690LP | 740/35 | BUV737 | BUV737 |
770LP | 820/60 | BUV805 | BUV805 |