Main ContentMariia Stefanenko, MSc
PhD-student
Citizenship: Ukraine
Education
2015 – 2017
M.Sc. in Molecular Biotechnology,
National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Kyiv, Ukraine
2010 – 2014
B.Sc. in Molecular Biotechnology,
National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Kyiv, Ukraine
Research Experience:
12/2021 – Present
Graduate student (Dr. Palygin Lab), Department of Medicine, Division of Nephrology, Medical University of South Carolina, Charleston, SC
11/2018 – Present
Graduate student, Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine
07/2019 – 11/2021
Cell biologist, Biotechnological laboratory, Vitality Medical & Research Center, Kyiv, Ukraine
Professional affiliations
2022 – Present
Member, American Society of Nephrology (ASN) 2018 – Present Member, Ukrainian Biophysics Society
2018 – Present
Member, Ukrainian Physiological Society
Professional training
2022
Confocal Microscopy for the Biosciences Cell & Molecular Imaging, Medical University of South Carolina, Charleston, SC.
2022
Use of Seahorse Analysis for renal tubular cells. Department of Drug Discovery and Biomedical Sciences at the Medical University of South Carolina, Charleston, SC.
2022
Surgeon for Rodent Survival Surgery. Division of Laboratory Animal Resources (DLAR) at the Medical University of South Carolina, Charleston, SC.
2018
IV International School of Biomedical Research "Study of the properties of stem cells of different genesis in models of nerve tissue damage", Bogomoletz Institute of Physiology, Kyiv, Ukraine.
2018
Practical course "Methods of culturing cells", Department of Neuromuscular Physiology, Bogomoletz Institute of Physiology, Kyiv, Ukraine.
2015
Practical course "Comparative sensitivity of cell cultures to clinical isolates of viruses", Shupyk National Medical Academy of Postgraduate Education, Department of Virology, Kyiv, Ukraine.
Personal statement
During my initial training at the Bogomoletz Institute of Physiology, Kyiv, Ukraine, in Dr. Isaev’s Laboratory, I got extensive experience studying molecular cell and ion channel physiology. I recently relocated to our long-standing collaborator and colleague Dr. Palygin’s lab due to the current war conflict in Ukraine. I continue to pursue my graduate degree under the supervision of Dr. Palygin in the Division of Nephrology, Medical University of South Carolina, Charleston. My work focuses on chronic kidney disease and glomerular function. Particularly, I am studying the role of GPCR and resident ion channels signaling in podocytes, mesangial, and parietal epithelial cells and how these pathways changed under pathophysiological conditions like diabetes and systemic lupus erythematosus. I routinely use sophisticated methods like cell culture, animal work, western blotting, immunocytochemistry, and confocal microscopy to study renal function in health and disease.
Areas of Expertise:
Electrophysiology (Neuroscience & Renal Physiology)
- Patch-clamp electrophysiology (freshly isolated renal tubules, cell culture, brain slices, isolated neurons)
- Extracellular field potential recordings (LTP, LTD, short-term plasticity)
- In vitro epilepsy modeling
Imaging
- Confocal microscopy
- Intracellular calcium and NO imaging
- Immunohistochemistry
Animal Surgery
- Surgical procedures for the implantation of electrodes and recording of EEGs.
- Implantation of telemetry systems.
- Kidney or brain perfusion.
Cell culture
- Culture maintenance.
- Cryobanking.
- Preparation of nutrient media.
- Work with primary and secondary cultures of stem cells, hPods, HMRC, OK, cardiomyocytes and HEK.
Behavior examination (rodents)
- Open field, Elevated plus maze, Fear conditioning, Social behavior, Morris water maze.
Other
- Vibrodissociation
- Western blotting
- Isolation and purification of DNA and RNA
- Polymerase chain reaction
Publications:
https://www.ncbi.nlm.nih.gov/myncbi/mariia.stefanenko.1/bibliography/public/
- Semenikhina M, Fedoriuk M, Stefanenko M, Klemens CA, Solanki A, Lipschutz JH, Staruschenko A, Palygin O, β-arrestin pathway modulates calcium influx in podocytes, in submission
- Colvert A, Semeikhina M, Stefanenko M, DeLeon-Pennell KY, Palygin O, and Van Beusecum JP, Hypertensive and Inflammatory Stimuli Regulate the Immunological Synapse Interface of Renal Endothelial Cells, in preparation
- Bohovyk R, Khedr S, Stefanenko M, Semenikhina M, Kravtsova O, Isaeva E, Klemens CA, Levchenko V, Palygin O, Staruschenko A, Heterozygous mutation of Protease-activated Receptor 1 attenuates the development of albuminuria and glomerular damage in type 2 diabetic nephropathy rats, in preparation
- Semenikhina M, Stefanenko M, Spires DR, Ilatovskaya DV, Palygin O. Nitric-Oxide-Mediated Signaling in Podocyte Pathophysiology. Biomolecules. 2022 May 25;12(6):745. doi: 10.3390/biom12060745. PMID: 35740870 - cover image
- Fedoriuk M, Stefanenko M, Bohovyk R, Cherninskyi A, Maximyuk O, Isaev D, Platonov M, Krishtal O. Pharmacological blockade of acid sensing ion channels normalizes emotionally conditioned learning disturbed due to epileptic status Fiziol. Zh. 2020; 66(5): 11-16. https://doi.org/10.15407/fz66.05.011
Oral talks:
Nitric oxide synthases and their role in diabetes. Division of Nephrology, Medical University of South Carolina, May 27, 2022, Charleston, SC
Posters:
- Stefanenko M, Semenikhina M, Fedoriuk M, Bohovyk R, Klemens CA, Solanki A, Lipschutz JH, Staruschenko A, Palygin O. RAS-mediated crosstalk between calcium and nitric oxide in podocyte. 15th Annual Department of Medicine Virtual Research Symposium. 2022, Charleston, SC.
- Semenikhina M, Stefanenko M, Fedoriuk M, Palygin O. Control of Renal Function in Health and Disease, South Carolina Smart State meeting 2022, Columbia, SC.
- Stefanenko M, Fedoriuk M, Bogovyk R, Semenikhina M, Maximyuk O, Krishtal O, Isaev D. Effects of selective antagonist of ASICs in the lps animal model of Parkinson’s disease, 2018, VII International Congress of Ukrainian Society for Biophysics, Ukraine.
- Stefanenko M, Fedoriuk M, Bogovyk R, Semenikhina M, Maximyuk O, Krishtal O, Isaev D. ASICs inhibition shifts affective behavior in the rat’s model of Parkinson’s disease. 35th Ernst Klenk Symposium in Molecular Medicine Rare diseases: From mechanisms to therapy and beyond, 2019, Cologne, Germany, meeting abstract, P 35, poster B-07.
- Demchenko S, Stefanenko M, Isaev D. Effect of proepileptic drugs on epileptiform activity in rat hippocampus following Parkinson’s disease. 35th Ernst Klenk Symposium in Molecular Medicine Rare diseases: From mechanisms to therapy and beyond, 2019, Cologne, Germany.
- Semenikhina M, Romanov A, Fedoriuk M, Stefanenko M, Isaev D, Isaeva O, Maximyuk O, and Krishtal O. Regulation of spontaneous synaptic activity in the lateral amygdala by acid- sensing ion channels. 100 years of microglia, 2019 Lausanne, Switzerland.
- Semenikhina M, Romanov A, Fedoriuk M, Stefanenko M, Isaev D, Maximyuk O, and Krishtal O. Acid-sensing ion channels regulate spontaneous activity in the amygdala. VIII annual meeting of Ukrainian Biophysical Society, 2019 Kiyv, Ukraine, meeting abstracts, P. 21.
- Stefanenko M, Fedoriuk M, Bogovyk R, Semenikhina M, Maximyuk O,Krishtal O, Isaev D. Effects of ASICs inhibition in animal model of Parkinson’s disease. Fiziologichnyi Zhurnal, 2019, 65 (S3), C. 18
- Savotchenko AV, Tkachenko YM, Drevytska TI, Morhachov RO, Demchenko SO, Stefanenko MV, Isaev DS. Development of experimental model of ARDS in SHR strain old rats. XV IMBG all-Ukrainian Conference of Young Scientists with international participation. 2021, Kyiv, Ukraine.
- Savotchenko A., Tkachenko Y., Drevytska T., Demchenko S., Stefanenko M., Morhachov R., Isaev D. The role of transient potential receptors in the development of acute respiratory distress syndrome FENS Regional Meeting, 2021, Krakow, Poland.
- Savotchenko A, Tkachenko Y, Drevytska T, Demchenko S, Stefanenko M, Morhachov R, Isaev Impact of virus sialidase blockage on synaptic plasticity in rat brain. 50th Neuroscience Meeting, 2021, Chicago, USA.