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Josie Silvaroli

silvaroli.1@buckeyemail.osu.edu

www.linkedin.com/in/josiesilvaroli

Columbus, OH

(440) 897-8510

ACADEMIC DEGREES

2020 – Present
The Ohio State University
Doctor of Philosophy, Pharmaceutics and Pharmacology
Columbus, Ohio 

2015 – 2019
Case Western Reserve University
Bachelor of Science, Chemistry
Cleveland, Ohio

2013 – 2015
Lakeland Community College
Associate of Applied Science, Biotechnology
Kirtland, Ohio

WORK EXPERIENCE

2020 – Present
Ohio State University, Navjot Pabla
Columbus, Ohio
Graduate Research Associate

Research focused on studying molecular pathways that are causal for acute kidney injury as well as potential therapeutic targets

  • Study protein expression and stability in various models of AKI
  • Generate novel transgenic mice and use established mouse AKI model to understand molecular pathways involved in pathogenesis.
2015 – 2019
Case Western Reserve University, Marcin Golczak
Cleveland, Ohio
Research Assistant II

Research focused on delineating vitamin A metabolism pathways within the visual cycle.

  • Conducted research project focused on determining protein confirmations, and kinetic parameters with different ligands.
  • Established a high-throughput screening methodology that allowed for determining interactions for cellular retinol-binding proteins.
2013 – 2015
Lakeland Community College
Kirtland, Ohio
Chemistry Student Lab Assistant
  • Analyze chemical compounds using instruments such as IR, NMR
  • Help a diverse group of students in understanding lab material

FELLOWSHIPS

Jan 2022 – Dec 2023

American Heart Association Predoctoral Fellowship

"The role of Cdkl5 in DNA damage response and acute kidney injury"

PUBLICATIONS

  1. Calderon RM, Smith CA, Miedzybrodzka EL, Silvaroli JA, Golczak M, Gribble FM, Reimann F, Blaner WS.Intestinal Enteroendocrine Cell Signaling: Retinol-binding Protein 2 and Retinoid Actions. Endocrinology. 2022 Jul 1;163(7):bqac064. doi: 10.1210/endocr/bqac064. PMID: 35552670; PMCID: PMC9162388
  2. Eisenmann ED, Garrison DA, Talebi Z, Jin Y, Silvaroli JA, Kim JG, Sparreboom A, Savona MR, Mims AS, Baker SD. Interaction of Antifungal Drugs with CYP3A- and OATP1B-Mediated Venetoclax Elimination. Pharmaceutics. 2022 Mar 23;14(4):694. doi: 10.3390/pharmaceutics14040694. PMID: 35456528; PMCID: PMC9025810.
  3. Zavorka Thomas ME, Jeon JY, Talebi Z, Buelow DR, Silvaroli J, Campbell MJ, Sparreboom A, Pabla N, Baker SD. Gilteritinib-induced upregulation of S100A9 is mediated through BCL6 in acute myeloid leukemia. Blood Adv. 2021 Dec 14;5(23):5041-5046. doi: 10.1182/bloodadvances.2021005614. PMID: 34614509; PMCID: PMC9153019.
  4. Bai Y, Kim JY, Bisunke B, Jayne LA, Silvaroli JA, Balzer MS, Gandhi M, Huang KM, Sander V, Prosek J, Cianciolo RE, Baker SD, Sparreboom A, Jhaveri KD, Susztak K, Bajwa A, Pabla NS. Kidney toxicity of the BRAF-kinase inhibitor vemurafenib is driven by off-target ferrochelatase inhibition. Kidney Int. 2021 Dec;100(6):1214-1226. doi: 10.1016/j.kint.2021.08.022. Epub 2021 Sep 15. PMID: 34534550; PMCID: PMC8608726.
  5. Silvaroli JA, Plau J, Adams CH, Banerjee S, Widjaja-Adhi MAK, Blaner WS, Golczak M. Molecular basis for the interaction of cellular retinol binding protein 2 (CRBP2) with nonretinoid ligands. J Lipid Res. 2021;62:100054. doi: 10.1016/j.jlr.2021.100054. Epub 2021 Feb 23. PMID: 33631211; PMCID: PMC8010219.
  6. Lee SA, Yang KJZ, Brun PJ, Silvaroli JA, Yuen JJ, Shmarakov I, Jiang H, Feranil JB, Li X, Lackey AI, Krężel W, Leibel RL, Libien J, Storch J, Golczak M, Blaner WS. Retinol-binding protein 2 (RBP2) binds monoacylglycerols and modulates gut endocrine signaling and body weight. Sci Adv. 2020 Mar 11;6(11):eaay8937. doi: 10.1126/sciadv.aay8937. PMID: 32195347; PMCID: PMC7065888.
  7. Widjaja-Adhi MAK, Silvaroli JA, Chelstowska S, Trischman T, Bederman I, Sayegh R, Golczak M. Deficiency in Acyl-CoA:Wax Alcohol Acyltransferase 2 causes evaporative dry eye disease by abolishing biosynthesis of wax esters. FASEB J. 2020 Oct;34(10):13792-13808. doi: 10.1096/fj.202001191R. Epub 2020 Aug 26. PMID: 32851726; PMCID: PMC7722226.
  8. Silvaroli JA, Widjaja-Adhi MAK, Trischman T, Chelstowska S, Horwitz S, Banerjee S, Kiser PD, Blaner WS, Golczak M. Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1. ACS Chem Biol. 2019 Mar 15;14(3):434-448. doi: 10.1021/acschembio.8b01070. Epub 2019 Feb 18. PMID: 30721022; PMCID: PMC6420351.
  9. Silvaroli JA, Pleshinger MJ, Banerjee S, Kiser PD, Golczak M. Enzyme That Makes You Cry-Crystal Structure of Lachrymatory Factor Synthase from Allium cepa. ACS Chem Biol. 2017 Sep 15;12(9):2296-2304. doi: 10.1021/acschembio.7b00336. Epub 2017 Jul 26. PMID: 28708375; PMCID: PMC5693239.
  10. Arne JM, Widjaja-Adhi MAK, Hughes T, Huynh KW, Silvaroli JA, Chelstowska S, Moiseenkova-Bell VY, Golczak M. Allosteric modulation of the substrate specificity of acyl-CoA wax alcohol acyltransferase 2. J Lipid Res. 2017 Apr;58(4):719-730. doi: 10.1194/jlr.M073692. Epub 2017 Jan 17. PMID: 28096191; PMCID: PMC5392747.
  11. Chelstowska S, Widjaja-Adhi MAK, Silvaroli JA, Golczak M. Impact of LCA-Associated E14L LRAT Mutation on Protein Stability and Retinoid Homeostasis. Biochemistry. 2017 Aug 29;56(34):4489-4499. doi: 10.1021/acs.biochem.7b00451. Epub 2017 Aug 15. PMID: 28758396; PMCID: PMC5682948.
  12. Silvaroli JA, Arne JM, Chelstowska S, Kiser PD, Banerjee S, Golczak M. Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures. J Biol Chem. 2016 Apr 15;291(16):8528-40. doi: 10.1074/jbc.M116.714535. Epub 2016 Feb 21. PMID: 26900151; PMCID: PMC4861425.
  13. PATENT: Marcin Bernard Golczak, Josie Ann Silvaroli. Cellular retinoid binding protein antagonists and uses thereof, U.S. Patent App. Serial No. 15734781 Published 2021/7/29.
  14. REVIEW: Chelstowska S, Widjaja-Adhi MA, Silvaroli JA, Golczak M. Molecular Basis for Vitamin A Uptake and Storage in Vertebrates. Nutrients. 2016 Oct 26;8(11):676. doi: 10.3390/nu8110676. PMID: 27792183; PMCID: PMC5133064.

PRESENTATIONS

2021

Josie A. Silvaroli, Ji Young Kim, Navjot Pabla. Role of off-target Ferrochelatase Inhibition in vemurafenib nephrotoxicity. American Society of Nephrology. Nov. 2021. (Oral presentation)

2021

Josie A. Sivaroli, Yuntao Bai, Ji Young Kim, Laura A. Jayne, Megha Gandhi, Kevin M. Huang, Veronika Sander, Jason Prosek, Kenar D. Jhaveri, Sharyn D. Baker, Alex Sparreboom, Amandeep Bajwa, Navjot Singh Pabla. Nephrotoxicity of the Braf-kinase inhibitor vemurafenib is driven by off-target ferrochelatase inhibition. Presented at: College of Pharmacy Research Day, OSU, 2021 (Virtual Poster presentation)

2020

Josie A. Silvaroli, Eric D. Eisenmann, Dominique Garrison, Alex Sparreboom, Sharyn D. Baker. Evaluating the impact of antifungal agents on OATP1B1. Divisions of Pharmaceutics and Pharmacology Scientific Virtual Retreat, OSU. 2020. (Virtual Poster presentation)

2017

Josie A. Silvaroli, William S. Blaner, Marcin Golczak. CRBP2 reveals binding affinity for endocannabinoid ligands not related with vitamin A metabolism. Presented at: Pharmacology Symposium, Kalahari, Ohio. 2017. (Poster presentation)

AWARDS

2018 – Scholarship, Iota Sigma Pi Frank Hovorka Prize, CWRU

2018 – Scholarship, The Outstanding Junior Award of Art and Sciences, CWRU

2016 – Scholarship, The Carl F. Prutton Prize, CWRU

2014 – Chemical Technician Certificate, LCC

2013 – Award, Outstanding Chemistry Student, LCC 2013 Scholarship, Lakeland Community College’s Board of Trustees Scholarship, LCC

SOCIETIES

2021 – Present Phi Kappa Phi Invited Member

2021 – Present American Society of Nephrology Member

2021 – Present American Heart Association Member

SERVICE

2022 – Present
Generation RX (COSI)
Volunteer
Teach and design creative activities for children to learn about medication safety and as well as about the science behind medicine working in our bodies

2020
Charitable Pharmacy of Central Ohio
Volunteer
Assisted physicians during patient examinations by confirming medicine recommendations and counseled patients on new medications with preceptor supervision

2019 – 2020
Helping Hands and Wellness Center
Volunteer
Quickly filled prescriptions and ensured accuracy for dispensing and organized medications

MENTORING

Undergraduate Students (Mentor):

Matthew Pleshinger – BS candidate, Youngstown State University (SU 2016)
• Currently a PhD Candidate in Biochemistry and Molecular Biology at Case Western Reserve University

Samantha Horwitz – BS candidate (Major Chemistry), Kent State University (SU 2018)
• Currently a PhD Candidate in Biochemistry at Emory College

Bhavya Girotra – BS Candidate (Major BSPS) (AU 2021- SU 2022)

Highschool Student (Mentor):

Randy Radiarta – (AU 2018 – SPR 2019)
• Currently a BS candidate (Major Civil and Environmental Engineering) at Case Western Reserve University

Medical Student (Mentor):

Thomas Trischman – M.D. Candidate, Case Western Reserve University (AU 2018 – SP 2019)
• Currently doing a Residency in Dermatology at the Mayo Clinic