Mass Spectrometry Core Facility (MSCF)
The mission of the Mass Spectrometry Core Facility (MSCF) is to serve as a nucleus to develop research and collaborations to increase and enhance competitiveness in biomedical discovery for researchers at the University of Mississippi Medical Center and across Mississippi.
The MSCF provides lipidomics, quantitative peptidomics/proteomics and metabolomics capabilities to researchers at UMMC as well as to other researchers from intrastate as well as interstate locales. The MSCF provides the expertise in LC/MS/MS, HPLC, sample preparation, method/assay development and data analysis and interpretation. The LC/MS/MS systems are very robust and allow not only targeted analyte quantitation but also discovery "-omics" when the biomolecules of interest are enriched (IP's, Off-line fractionation/enrichment, gel bands).
The MSCF is equipped with two ABSCIEX QTRAP mass spectrometers (Models 4000 and 5500, ABSCIEX, Foster City, CA) with TurboV Electrospray ion (ESI) sources each coupled to Dionex Ultimate 3000 HPLC systems under the control of Analyst 1.5.2 software. They have been optimized specifically for quantitative measurement of many physiologically relevant analytes at very low limits of detection. In addition, 2 HPLC systems (an Agilent 1100 series and one Dionex Ultimate system) allow measurement of more abundant analytes and provide sample prep, cleanup, and enrichment capability prior to LC/MS/MS analysis.
Current methods/assays available
- Sex Steroids and Other Endocrine Hormones
- Angiotensin II, Ang 1-7, and other RAAS Peptides
- Drugs and Xenobiotics for PK, Stability, and Metabolite Studies
- Prostaglandins /Isoprostanes
- Lysophosphatidic acid
- Protein Quantification (MRMs)
- Lipid Quant/Lipidomics
- Discovery Proteomics on IP pulldowns and Gel Bands
Stanley V. Smith, PhD
Gary R. Bishop, PhD
Michael Garrett, PhD
Babbette LaMarca, PhD
- UMMC users - Refer to intranet
We welcome collaboration with external users. Contact us by phone or email.