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Sputum

• Performing lab: Cytopathology (N018) Ext: 4-1550 
• Availability: 8 a.m.-5 p.m. Monday-Friday
• Turnaround time: One to two working days. 
• Specimen type: Sputum  
• Container: Clean container with tight-fitting lid. 
• Volume: 2-6 mL
  • Minimum volume: 2 mL

Special instructions

• Collect sample in morning upon rising.
• Specimen transport requirements
• Cytologic specimens should be labeled with printed barcode labels (do not put label on tops)
• Placed in a biohazard bag and transported with the printed requisition to the Cytology Laboratory, N018
• Logged in and placed on the top shelf of the refrigerator

Patient preparation

• Rinse mouth out with water before attempting to bring up sputum.
• Cough deeply.
• Deposit secretion coughed up from deep within bronchial tree into container.
• Close container tightly, label on the body of the container (not the lid) and place in a biohazard bag.

Causes for rejection

• Inadequate identification
• Improper fixation
• Specimen type not identified

More information

• Complete the Non-Gyn cytology order in Epic to include pertinent history, prior diagnostic/therapeutic procedures, X-ray, clinical findings, and results of prior surgeries.
• Outreach facilities should use the preprinted “University Outreach Pathology Requisition” and submit with the sample.
• Indicate request for additional special stains or other procedures.
• Indicate if body fluid precautions are necessary.