Always consult with the Proteomics Core manager prior to submitting (submission form) your samples for analysis. This will ensure that we understand your specific needs before submission of samples.
1x Protease Inhibitor Cocktail (Roche cOmplete Mini with EDTA, ordered from Fisher #50-720-4060): Solubilize 1 tablet in 1 mL PBS to make a 10x solution. All buffers have to contain 1x PI final concentration.
Reagent 4: urea, thiourea, Trizma, detergent C7BzO
To dissolve: 1. Add 15 ml dH2O to bottle and swirl, 2. Heat to 30°C for 30-60 min, until dissolved, 3. Use fresh or aliquot (1ml/tube) and store at -20°C
8M Urea with 1M ammonium bicarbonate: