Jonathan P. Hosler, PhD

Jonathan P. Hosler, PhD

Professor

Department:
SOM-Cell and Molecular Biology

Email:
jhosler@umc.edu

Work Phone:
(601) 984-1861

Education

Michigan State University, Postdoctoral, Biochemistry1987
University of Michigan, PhD, Biology1983
Duke University, Postdoctoral, Biology1983
Ohio Wesleyan University, BA, Botany & Bacteriology1976

Current Positions

Professor, School of Medicine, Department of Biochemistry2005 - Present
The University of Mississippi Medical Center
Jackson, Mississippi 39216

Academic Appointments or Other Previous Appointments

Associate Professor, Univerisity of Mississippi Medical Center, School of Medicine, Department of Biochemistry2000 - 2000
Assistant Professor, University of Mississippi Medical Center, School of Medicine, Department of Biochemistry1995 - 2000
Research Assistant Professor/Research Associate, Michigan State University, School of , Department of Biochemistry1987 - 1994
PostDoc, Duke University, School of , Department of Division of Biology1983 - 1987

Sponsored Program Funding

Extramural, Nuclear and mitochondrial genome crosstalk in kidney, NIH08/2021 - 07/2026
Extramural, Physiological, cellular and molecular metabolic consequences of gestational diabetes, NIH08/2021 - 07/2026
Extramural, Renal Mechanisms of Hypertension in Autoimmune Disease, Veterans Administration04/2020 - 03/2024
Extramural, COBRE: Center of Excellence in Perinatal Research, NIH06/2018 - 05/2020
Extramural, Molecular Basis of Post-Ischemic Maladaption in the Insulin Resistant Heart, NIH04/2018 - 03/2019
Extramural, Supplement to The Role of Subunit III in Cytochrome Oxidase Function(R01 GM056824-07S1), National Institutes of Health, Institute of General Medicine08/2005 - 07/2007
Extramural, The Role of Subunit III in Cytochrome Oxidase Function, NIH GM01/2004 - 12/2008
Extramural, The Molecular Basis for Metabolic and Energetic Diversity. (DE-FG02-01ER63232), U.S. Department of Energy, Office of Biological and Environmental Research09/2001 - 09/2004
Extramural, The Role of Subunit III in Cytochrome Oxidase Function (R01 GM56824), National Institutes of Health, Institute of General Medicine06/1998 - 05/2003
Extramural, Suicide Inactivation of Cytochrome c Oxidase: A Major Source of Declining Energy Metabolism in the Aging Heart (9730167N), Scientist Development Grant, American Heart Association01/1998 - 12/2001
Extramural, A Model System to Study the Molecular Mechanisms of Mitochondrial Disease Affecting Heart Function (MS-G-960072), Grant-in-Aid, American Heart Association, Mississippi Affiliate07/1996 - 06/1998

Publications

Journal Article

Booz GW, Kennedy D, Bowling M, Robinson T, Azubuike D, Fisher B, Brooks K, Chinthakuntla P, Hoang NH, Hosler JP, Cunningham Jr, MW Angiotensin II type 1 receptor agonistic autoantibody blockade improves postpartum hypertension and cardiac mitochondrial function in rat model of preeclampsia Biol. Sex Differ. 2021 12:582021
Hoang, NH, Strogolova, V, Mosley, JJ, Stuart, RA and Hosler, J Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV J. Biol. Chem., 2019, 294(46):17669-176772019
Alnajjar KS, Hosler JP, Prochaska L The role of the N-Terminus of subunit III in proton uptake in cytochrome c oxidase of Rhodobacter sphaeroides Biochemistry. 2014; 53:496-5042014
Zouein F, Duhe R, Arany I, Shirey K, Hosler JP, Liu H, Iman Saad I, Kurdi M, Booz G Loss of STAT3 in mouse embryonic fibroblasts reveals its Janus-like actions on mitochondrial function and cell viability. Cytokine. 2014 66:7-162013
Cleary JD, Stover K, Farley J, Daley W, Kyle PB, Hosler J Cardiac Toxicity of Azole Antifungals Pharmacology & Pharmacy. 2013; 4:362-3682013
Johansson AL, Carlsson J, Högbom M, Hosler JP, Gennis RB, Brzezinski, P Proton uptake and pKa changes in the uncoupled Asn139Cys variant of cytochrome c oxidase Biochemistry. 2013; 52:827-362013
Ouyang H, Han H, Roh JH, Hemp J, Hosler JP, Gennis, RB Functional importance of a pair of conserved glutamic acid residues and of Ca2+ binding in the cbb3-type oxygen reductases from Rhodobacter sphaeroides and Vibrio cholera. Biochemistry. 2012; 51:7290-72962012
Huo L, Fielding AJ, Chen Y, Li T, Iwaki H, Hosler JP, Chen L, Hasegawa Y, Que L, Liu A Evidence for a dual role of an active site histidine in α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase Biochemistry. 2012; 51:5811-58212012
L. Varanasi, J.P. Hosler Subunit III-depleted cytochrome c oxidase provides insight into the process of proton uptake by proteins. Biochim. Biophys. Acta 1817: 545-512012
Thompson, A.K., Gray, J., Liu, A. and Hosler, J.P The roles of Rhodobacter sphaeroides copper chaperones PCuAC and Sco (PrrC) in the assembly of the copper centers of the aa3-type and the cbb3-type cytochrome c oxidases. Biochim. Biophys Acta 1817:955-9642012
Varanasi, L. and Hosler, J.P Alternative initial proton acceptors for the D pathway of Rhodobacter sphaeroides cytochrome c oxidase Biochemistry 50: 2820-28282011
Egawa T., Ganesan K., Lin M.T., Yu M.A., Hosler J.P., Yeh S.R., Rousseau D.L. and Gennis R.B. Differential effects of glutamate-286 mutations in the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides and the cytochrome bo3 ubiquinol oxidase from Escherichia coli. Biochim. Biophys. Acta 1807: 1342-82011
Thompson A.K., Smith, D., Gray, J., Carr, H.S., Liu, A., Winge, D.R., and Hosler, J.P. Mutagenic analysis of Cox11 of Rhodobacter sphaeroides: insights into the assembly of CuB of cytochrome c oxidase. Biochemistry 49: 5651-612010
Egawa T., Lin M.T., Hosler J.P., Gennis R.B., Yeh S.R., Rousseau D.L Communication between R481 and CuB in cytochrome bo3 ubiquinol oxidase from Escherichia coli. Biochemistry 48:12113-24.2009
Ma, J.K., Lee, S., Choi, M., Bishop, G.R., Hosler, J.P. and Davidson, V.L. The axial ligand and extent of protein folding determine whether Zn or Cu binds to amicyanin. J. Inorg. Biochem. 2008; 102: 342-3462007
Li, T., Ma, J.K., Hosler, J.P., Davidson, V.L. and Liu, A. Detection of transient intermediates in the metal-dependent nonoxidative decarboxylation catalyzed by alpha-amino-beta-carboxymuconate-ε-semialdehyde decarboxylase. J. Amer. Chem. Soc. 2007; 129: 9278-92792007
Qin, L., Mills, D., Hiser, C., Murphree, A., Garavito, M.R., Ferguson-Miller, S. and Hosler, J. Crystallographic location and mutational analysis of the Zn/Cd inhibitory sites and the role of lipidic carboxylates in rescuing proton path mutants in cytochrome c oxidase. Biochemistry 46: 6239-62482007
Carrell, CJ, Ma, JK, Antholine, WE, Hosler, JP, Mathews, FS, and Davidson, VL. Generation of novel copper sites by mutation of the axial ligands of amicyanin. Atomic resolution structures and spectroscopic properties. Biochemistry 46: 1900-1912.2007
Megehee, J.A., Hosler, J.P. and Lundrigan, M.D. Evidence for a cytochrome bcc-aa3 interaction in the respiratory chain of Mycobacterium smegmatis Microbiology 152: 823-8292006
Varanasi, L., Mills, D., Murphree, A., Gray, J., Purser, C., Baker, R. and Hosler, J. Altering conserved lipid binding sites in cytochrome c oxidase of Rhodobacter sphaeroides perturbs the interaction between subunits I and III and promotes suicide inactivation of the enzyme. Biochemistry 45: 14896-149072006
Adelroth, P and Hosler, J.P Surface proton donors for the D-pathway of cytochrome c oxidase in the absence of subunit III Biochemistry 45: 8308-83182006
Hosler, J.P., Ferguson-Miller, S. And Mills, D.A. Energy Transduction: Proton Transfer through the Respiratory Complexes. Ann. Rev. Biochem. 75:165-187.2006
Mills, D.A. and Hosler, J.P. Slow Proton Transfer through the Pathways for Pumped Protons in Cytochrome c Oxidase Induces Suicide Inactivation of the Enzyme Biochemistry 44:4656-4666.2005
Smith, D., Gray, J., Mitchell, L. Antholine, W.E. and Hosler, J.P. Assembly of Cytochrome c Oxidase in the Absence of the Assembly Protein Surf1p Leads to Loss of the Active Site Heme. J. Biol. Chem. 280: 17652-17656.2005
Hosler, J.P. The influence of subunit III of cytochrome c oxidase on the D pathway, the proton exit pathway and mechanism-based inactivation in subunit I Biochem. Biophys. Acta-Bioenergetics 1655: 332-339.2004
Bratton, M.R., Mills, D, Castleden, C.K., Hosler, J. and Muenier, B Disease-related mutations in cytochrome c oxidase studied in yeast and bacterial models. Eur. J. Biochem. 270: 1-9.2003
Mills, D.A., Tan, Z., Ferguson-Miller, S. and Hosler, J.P. A role for subunit III in proton uptake into the D pathway and a possible proton exit pathway in Rhodobacter sphaeroides cytochrome c oxidase. Biochemistry 42: 7410-7417.2003
Gilderson, G., Salamonsson, L., Aagaard, A, Gray, J., Brzezinski, P. and Hosler, J.P. Subunit III of cytochrome c oxidase of Rhodobacter sphaeroides is required to maintain rapid proton uptake through the D pathway at physiologic pH. Biochemistry 42:7400-74092003
Hosler, J.P and Shapleigh, J.P. Principles of Aerobic Respiration The Encylopedia of Environmental Microbiology, Gabriel Bitton, ed., John Wiley & Sons, New York.2002
Hiser, L and Hosler, J.P. Heme A is not essential for assembly of the subunits of cytochrome c oxidase of Rhodobacter sphaeroides J. Biol. Chem 276: 45403-454072001
Mackenzie, C., Choudhary, M., Larimer, F., Predki, P.F., Stilwagen, S., Armitage, J.P., Barber, R.D., Donohue, T.J., Hosler, J.P., Newman, J.E., Shapleigh, J.P., Sockett, R.E., Zeilstra-Ryalls, J. and Kaplan, S. The home stretch, a first analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1. Photosynthesis Research 70: 19-412001
Hiser, L., DiValentin, M. and Hosler, J. Cox11p Is Required for Stable Formation of the CuB and Magnesium Centers of Cytochrome c Oxidase J. Biol. Chem. 275: 619-623.2000
Bratton, M.R., Hiser, L., Antholine, W., Hoganson, C. and Hosler, J.P. Identification of the structural subunits required for formation of the metal centers in subunit I of cytochrome c oxidase of Rhodobacter sphaeroides. Biochemistry 39: 12989-129952000
Bratton, M., Pressler, M. and Hosler, J Suicide Inactivation of Cytochrome c Oxidase: Catalytic Turnover in the Absence of Subunit III Alters the Active Site Biochemistry 38: 16236-16245.1999
Graichen, M.E., Jones, L.H., Sharma, B., van Spanning, R.J.M., Hosler, J.P. and Davidson, V.L. Heterologous Expression of Correctly Assembled Methylamine Dehydrogenase in Rhodobacter sphaeroides J. Bacteriology 181: 4216-42221999
Mathews, F.S., Chen, Z.-W., Durley, R.C.E., Davidson, V.L., Jones, L.H., Graichen, M.E., Hosler, J., Merli, A., Broderson, D.E. and Rossi, G.L. Structural Research on the Methylamine Dehydrogenase Redox Chain of Paracoccus denitrificans Biological Electron Transfer Chains: Genetics, Composition and Mode of Operation, Canters, G.W and Vijgenboom, eds., Kluwer Academic publishers, The Netherlands, pp. 129-146.1998
Zhen, Y., Follmann, K., Qian, J., Hayward, T., Nilsson, T., Dahn, M., Hamer, A.G., Hosler, J.P. and Ferguson-Miller, S Overexpression and Purification of Cytochrome c Oxidase from Rhodobacter sphaeroides. Protein Expression and Purification 13: 326-336.1998
Davidson, V.L., Jones, L.H., Graichen, M.E., Mathews, F.S. and Hosler, J.P. Factors Which Stabilize the Methylamine Dehydrogenase-Amicyanin Electron Transfer Protein Complex Revealed by Site-Directed Mutagenesis. Biochemistry 36:12733-127381997
Babcock, G.T., Dienum, J., Hosler, J. Kim, Y., Pressler, D., Proshlyakov, H., Schelvis, C., Varotsis, C. and Ferguson-Miller, S. Ligand Dynamics in the Binuclear Site in Cytochrome Oxidase. Oxygen Homeostasis and its Dynamics, Ishimura, Y, Shimada, H and Suematsu, M, Eds., Vol. 1, pp. 47-56, Springer-Verlag, Tokyo.1997
Mitchell, D.M., Adelroth, P., Hosler, J.P., Fetter, J., Brzezinski, P., Pressler, M., Aasa, R., Malmstrom, B., Alben, J.O., Babcock, G., Gennis, R.B. and Ferguson-Miller, S A Ligand Exchange Mechanism of Proton Pumping Involving Tyrosine-422 of Subunit I of Cytochrome Oxidase is Ruled Out Biochemistry 35:824-8281996
Hosler, J.P, Shapleigh, Mitchell, D.M., J.P., Kim, Y., Pressler, M., Georgiou, C., Babcock, G.T., Alben, J.O., Ferguson-Miller, S. and Gennis, R.B. Polar Residues in Helix VIII of Subunit I of Cytochrome c Oxidase Influence the Activity And Structure of the Active Site Biochemistry 35:10776-10783.1996
Wang, J., Takahashi, S., Rousseau, D., Hosler, J., Ferguson-Miller, S., Mitchell, D. and Gennis, R. Two Conformations of the Catalytic Site in the aa3-Type Cytochrome c Oxidase from Rhodobacter sphaeroides Biochemistry 34:9819-9825.1995
Fetter, J., Qian, J., Shapleigh, J., Thomas, J., Garcia-Horsman, A., Schmidt, E., Hosler, J., Babcock, G., Gennis, R. and Ferguson-Miller, S. A Proton Relay Pathway in Cytochrome c Oxidase. Proc. Natl. Acad. Sci. U.S.A. 92:1604-16081995
Espe, M., Hosler, J.P., Ferguson-Miller, S., Babcock, G.T. and McCracken, J. A CW– and Pulsed–EPR Characterization of the Mn2+ Site in Rhodobacter sphaeroides Cytochrome c Oxidase. Biochemistry 34:7593-7602.1995
Hosler, J.P., Espe, M.P., Zhen, Y., Babcock, G.T. and Ferguson-Miller, S Analysis of Site-Directed Mutants Locates a Non-Redox Active Metal Near the Active Site of Cytochrome c Oxidase of Rhodobacter sphaeroides Biochemistry 34:7586-7592.1995
Hosler, J.P., Kim. Y., Shapleigh, J.P., Alben, J.O., Gennis, R.B., Ferguson-Miller, S. and Babcock, G.T. Vibrational Characteristics of Mutant and Wild-Type Carbon-Monoxy Cytochrome c Oxidase: Support for the Current Model of the Trinuclear Center J. Am. Chem. Soc. 116:5515-55161994
Calhoun, M.W., Thomas, J.W., Hill, J.J., Hosler, J.P., Shapleigh, J.P., Tecklenburg, M.M.J., Ferguson-Miller, S., Babcock, G T., Alben, J.O. and Gennis, R.B Identity of The Axial Ligand of The High Spin Heme in Cytochrome Oxidase: Spectroscopic Characterization of Mutants in the bo-Type Oxidase of Eschericia coli and the aa3-Type Oxidase of Rhodobacter sphaeroides. Biochemistry 32: 10905-109111993
Hosler, J.P., S. Ferguson-Miller, M.W. Calhoun, J.W. Thomas, J. Hill, L. Lemieux, J. Ma, C. Georgiou, J. Fetter, J. Shapleigh, M.M.J. Tecklenburg, G.T. Babcock, R.B. Gennis Insight into the Active-Site Structure and Function of Cytochrome Oxidase by Analysis of Site-Directed Mutants of Bacterial Cytochrome aa3 and Cytochrome bo J. Bioenerg. Biomemb. 25:121-136. (featured as a ‘Hot Paper’ in The Scientist, February, 1995)1993
Hosler, J.P., Shapleigh, J.P., Tecklenburg, M.M.J., Thomas, J.W., Kim. Y., Espe, M., Fetter, J., Babcock, G.T., Alben, J.O., Gennis, R.B., and Ferguson-Miller, S. A Loop Between Transmembrane Helices IX and X of Subunit I of Cytochrome c Oxidase Caps the Heme a-Heme a3-CuB Center. Biochemistry 33: 1194-1201.1993
Cao, J., J. Hosler, J. Shapleigh, A. Revzin, and S. Ferguson-Miller Cytochrome aa3 of Rhodobacter sphaeroides as a Model for Mitochondrial Cytochrome c Oxidase: The CoxII/CoxIII Operon Codes for Structural and Assembly Proteins Homologous to Those in Yeast. J. Biol. Chem. 267:24273-242781992
Hosler, J.P., J. Fetter, M.M.J. Tecklenburg, M. Espe, C. Lerma and S. Ferguson-Miller Cytochrome aa3 of Rhodobacter sphaeroides as a Model for Mitochondrial Cytochrome c Oxidase: Purification, Kinetics, Proton Pumping and Spectral Analysis J. Biol. Chem. 267: 24262-24272.1992
Shapleigh, J., J. Hosler, M. Tecklenburg, Y. Kim, G. Babcock, R. Gennis and S. Ferguson-Miller Definition of the Catalytic Site of Cytochrome c Oxidase: The Specific Ligands of Heme a and the Heme a3-CuB Center Proc. Natl. Acad. Sci. USA 89:4786-4790.1992
Hosler, J.P. Biogenesis/Assembly of Respiratory Enzyme Complexes Biochim. Biophys. Acta 1817: 849-8502012
Strogolova V, Hoang NH, Hosler J, Stuart RA, The yeast mitochondrial proteins Rcf1 and Rcf2 support the enzymology of the cytochrome c oxidase complex and generation of the proton motive force. The Journal of biological chemistry, 2019 Mar 29;294(13):4867-48772019
Hoang NH, Strogolova V, Mosley JJ, Stuart RA, Hosler J, Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV. The Journal of biological chemistry, 2019 Nov 15;294(46):17669-176772019
Edwards KS, Ashraf S, Lomax TM, Wiseman JM, Hall ME, Gava FN, Hall JE, Hosler JP, Harmancey R, Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion. Basic research in cardiology, 2018 Oct 29;113(6):472018
Kahle M, Ter Beek J, Hosler JP, Ädelroth P, The insertion of the non-heme Fe(B) cofactor into nitric oxide reductase from P. denitrificans depends on NorQ and NorD accessory proteins. Biochimica et biophysica acta. Bioenergetics, 2018 Oct;1859(10):1051-10582018
Sun C, Benlekbir S, Venkatakrishnan P, Wang Y, Hong S, Hosler J, Tajkhorshid E, Rubinstein JL, Gennis RB, Structure of the alternative complex III in a supercomplex with cytochrome oxidase. Nature, 2018 May;557(7703):123-1262018
Timón-Gómez A, Nývltová E, Abriata LA, Vila AJ, Hosler J, Barrientos A, Mitochondrial cytochrome c oxidase biogenesis: Recent developments. Seminars in cell & developmental biology, 2018 Apr;76:163-1782018
Lawson WJ, Shirey K, Spann RA, Zamarripa CA, Hosler JP, Grayson BE, Vertical sleeve gastrectomy improves indices of metabolic disease in rodent model of surgical menopause. Menopause (New York, N.Y.), 2017 Apr;24(4):426-4362017
Shirey K, Stover KR, Cleary J, Hoang N, Hosler J, Membrane-Anchored Cyclic Peptides as Effectors of Mitochondrial Oxidative Phosphorylation. Biochemistry, 2016 Apr 12;55(14):2100-112016
Musatov A, Varhač R, Hosler JP, Sedlák E, Delipidation of cytochrome c oxidase from Rhodobacter sphaeroides destabilizes its quaternary structure. Biochimie, 2016 Jun;125:23-312016
Puskarich MA, Kline JA, Watts JA, Shirey K, Hosler J, Jones AE, Early alterations in platelet mitochondrial function are associated with survival and organ failure in patients with septic shock. Journal of critical care, 2016 Feb;31(1):63-72016
Munusamy S, do Carmo JM, Hosler JP, Hall JE, Obesity-induced changes in kidney mitochondria and endoplasmic reticulum in the presence or absence of leptin. American journal of physiology. Renal physiology, 2015 Oct 15;309(8):F731-432015
Beltrán J, Kloss B, Hosler JP, Geng J, Liu A, Modi A, Dawson JH, Sono M, Shumskaya M, Ampomah-Dwamena C, Love JD, Wurtzel ET, Control of carotenoid biosynthesis through a heme-based cis-trans isomerase. Nature chemical biology, 2015 Aug;11(8):598-6052015
Wikström M, Sharma V, Kaila VR, Hosler JP, Hummer G, New perspectives on proton pumping in cellular respiration. Chemical reviews, 2015 Mar 11;115(5):2196-2212015
Zouein FA, Duhé RJ, Arany I, Shirey K, Hosler JP, Liu H, Saad I, Kurdi M, Booz GW, Loss of STAT3 in mouse embryonic fibroblasts reveals its Janus-like actions on mitochondrial function and cell viability. Cytokine, 2014 Mar;66(1):7-162014
Alnajjar KS, Hosler J, Prochaska L, Role of the N-terminus of subunit III in proton uptake in cytochrome c oxidase of Rhodobacter sphaeroides. Biochemistry, 2014 Jan 28;53(3):496-5042014
DeCoursey TE, Hosler J, Philosophy of voltage-gated proton channels. Journal of the Royal Society, Interface, 2014 Mar 6;11(92):201307992014
Johansson AL, Carlsson J, Högbom M, Hosler JP, Gennis RB, Brzezinski P, Proton uptake and pKa changes in the uncoupled Asn139Cys variant of cytochrome c oxidase. Biochemistry, 2013 Feb 5;52(5):827-362013
Ouyang H, Han H, Roh JH, Hemp J, Hosler JP, Gennis RB, Functional importance of a pair of conserved glutamic acid residues and of Ca(2+) binding in the cbb(3)-type oxygen reductases from Rhodobacter sphaeroides and Vibrio cholerae. Biochemistry, 2012 Sep 18;51(37):7290-62012
Huo L, Fielding AJ, Chen Y, Li T, Iwaki H, Hosler JP, Chen L, Hasegawa Y, Que L Jr, Liu A, Evidence for a dual role of an active site histidine in α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase. Biochemistry, 2012 Jul 24;51(29):5811-212012
Thompson AK, Gray J, Liu A, Hosler JP, The roles of Rhodobacter sphaeroides copper chaperones PCu(A)C and Sco (PrrC) in the assembly of the copper centers of the aa(3)-type and the cbb(3)-type cytochrome c oxidases. Biochimica et biophysica acta, 2012 Jun;1817(6):955-642012
Varanasi L, Hosler JP, Subunit III-depleted cytochrome c oxidase provides insight into the process of proton uptake by proteins. Biochimica et biophysica acta, 2012 Apr;1817(4):545-512012
Egawa T, Ganesan K, Lin MT, Yu MA, Hosler JP, Yeh SR, Rousseau DL, Gennis RB, Differential effects of glutamate-286 mutations in the aa(3)-type cytochrome c oxidase from Rhodobacter sphaeroides and the cytochrome bo(3) ubiquinol oxidase from Escherichia coli. Biochimica et biophysica acta, 2011 Oct;1807(10):1342-82011
Varanasi L, Hosler J, Alternative initial proton acceptors for the D pathway of Rhodobacter sphaeroides cytochrome c oxidase. Biochemistry, 2011 Apr 12;50(14):2820-82011
Thompson AK, Smith D, Gray J, Carr HS, Liu A, Winge DR, Hosler JP, Mutagenic analysis of Cox11 of Rhodobacter sphaeroides: insights into the assembly of Cu(B) of cytochrome c oxidase. Biochemistry, 2010 Jul 13;49(27):5651-612010
Egawa T, Lin MT, Hosler JP, Gennis RB, Yeh SR, Rousseau DL, Communication between R481 and Cu(B) in cytochrome bo(3) ubiquinol oxidase from Escherichia coli. Biochemistry, 2009 Dec 29;48(51):12113-242009
Ma JK, Lee S, Choi M, Bishop GR, Hosler JP, Davidson VL, The axial ligand and extent of protein folding determine whether Zn or Cu binds to amicyanin. Journal of inorganic biochemistry, 2008 Feb;102(2):342-62008
Li T, Ma JK, Hosler JP, Davidson VL, Liu A, Detection of transient intermediates in the metal-dependent nonoxidative decarboxylation catalyzed by alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase. Journal of the American Chemical Society, 2007 Aug 1;129(30):9278-92007
Qin L, Mills DA, Hiser C, Murphree A, Garavito RM, Ferguson-Miller S, Hosler J, Crystallographic location and mutational analysis of Zn and Cd inhibitory sites and role of lipidic carboxylates in rescuing proton path mutants in cytochrome c oxidase. Biochemistry, 2007 May 29;46(21):6239-482007
Carrell CJ, Ma JK, Antholine WE, Hosler JP, Mathews FS, Davidson VL, Generation of novel copper sites by mutation of the axial ligand of amicyanin. Atomic resolution structures and spectroscopic properties. Biochemistry, 2007 Feb 20;46(7):1900-122007
Varanasi L, Mills D, Murphree A, Gray J, Purser C, Baker R, Hosler J, Altering conserved lipid binding sites in cytochrome c oxidase of Rhodobacter sphaeroides perturbs the interaction between subunits I and III and promotes suicide inactivation of the enzyme. Biochemistry, 2006 Dec 19;45(50):14896-9072006
Adelroth P, Hosler J, Surface proton donors for the D-pathway of cytochrome c oxidase in the absence of subunit III. Biochemistry, 2006 Jul 11;45(27):8308-182006
Megehee JA, Hosler JP, Lundrigan MD, Evidence for a cytochrome bcc-aa3 interaction in the respiratory chain of Mycobacterium smegmatis. Microbiology (Reading, England), 2006 Mar;152(Pt 3):823-8292006
Mills DA, Hosler JP, Slow proton transfer through the pathways for pumped protons in cytochrome c oxidase induces suicide inactivation of the enzyme. Biochemistry, 2005 Mar 29;44(12):4656-662005
Smith D, Gray J, Mitchell L, Antholine WE, Hosler JP, Assembly of cytochrome-c oxidase in the absence of assembly protein Surf1p leads to loss of the active site heme. The Journal of biological chemistry, 2005 May 6;280(18):17652-62005
Hosler JP, The influence of subunit III of cytochrome c oxidase on the D pathway, the proton exit pathway and mechanism-based inactivation in subunit I. Biochimica et biophysica acta, 2004 Apr 12;1655(1-3):332-92004
Mills DA, Tan Z, Ferguson-Miller S, Hosler J, A role for subunit III in proton uptake into the D pathway and a possible proton exit pathway in Rhodobacter sphaeroides cytochrome c oxidase. Biochemistry, 2003 Jun 24;42(24):7410-72003
Gilderson G, Salomonsson L, Aagaard A, Gray J, Brzezinski P, Hosler J, Subunit III of cytochrome c oxidase of Rhodobacter sphaeroides is required to maintain rapid proton uptake through the D pathway at physiologic pH. Biochemistry, 2003 Jun 24;42(24):7400-92003
Bratton M, Mills D, Castleden CK, Hosler J, Meunier B, Disease-related mutations in cytochrome c oxidase studied in yeast and bacterial models. European journal of biochemistry, 2003 Mar;270(6):1222-302003
Hosler JP, Ferguson-Miller S, Mills DA, Energy transduction: proton transfer through the respiratory complexes. Annual review of biochemistry, 2006;75:165-87

Review Article

DeCoursey TE, Hosler J Philosophy of voltage-gated proton channels J. R. Soc. Interface. 2014; 11, 201307992013

Book Chapter

Zannoni, D., Schoepp-Cothenet, B. and Hosler, J. Respiration and Respiratory Complexes. In: Hunter CN, Daldal, F, Thurnauer MC, and Beatty JT (eds) The Purple Phototrophic Bacteria. Advances in Photosynthesis and Respiration, Vol. 28, pp. 537-561 Springer, Dordrecht, The Netherlands, 20082009

Presentations

Telluride Science Research Center Workshop on Proton Transfer in Biology2010
Gordon Research Conference on Protons and Membrane Reactions2009
Max Plank Institute of Biophysics, Frankfurt, Germany2008
National and International Invited Seminars/Lectures, Copper chaperones in the assembly of cytochrome c oxidase, FASEB Research Conference on the Assembly of the Mitochondrial Respiratory Chain, Tucson, AZ08/2007
State/Local Invited Seminars/Lectures, Department of Physiology, UMMC2007
• Gordon Research Conference on Molecular and Cellular Bioenergetics2007
FASEB Research Conference on the Assembly of the Mitochondrial Respiratory Chain2007
State/Local Invited Seminars/Lectures, Spring Research Symposium 1995; 1998; 2002, 2007, Department of Biochemistry, Univerisity of Mississippi Medical Center2007
Gordon Research Conference on Protons and Membrane Reactions2006
National and International Invited Seminars/Lectures, Department of Biochemistry, Michigan State University2006
Radicals and Quinones, • Gordon Research Conference on Protein Derived Cofactors2006
National and International Invited Seminars/Lectures, Department of Chemistry, University of Utah2003
National and International Invited Seminars/Lectures, Department of Biochemistry, University of Utah2003
National and International Invited Seminars/Lectures, Rhodobacter sphaeroides, Genomes to Life Meetings, US Department of Energy, Sante Fe, Sante Fe2003
State/Local Invited Seminars/Lectures, Department of Chemistry, Jackson State University2002
State/Local Invited Seminars/Lectures, UMC Research Seminar Series2002
National and International Invited Seminars/Lectures, Department of Biochemistry, University of Texas, San Antonio2001
National and International Invited Seminars/Lectures, Rhodofest I, Rhodobacter sphaeroides Genome Conference, University of Texas Medical Center, Houston2001
National and International Invited Seminars/Lectures, Department of Biochemistry and Molecular Biology, Wright State University School of Medicine1999
State/Local Invited Seminars/Lectures, Department of Pharmacology and Toxicology, University of Mississippi Medical Center1998
State/Local Invited Seminars/Lectures, Department of Physiology, University of Mississippi Medical Center1998
State/Local Invited Seminars/Lectures, Department of Biology, Tougaloo College1997
State/Local Invited Seminars/Lectures, Department of Biochemistry, Mississippi State University1997
State/Local Invited Seminars/Lectures, Department of Chemistry, University of Mississippi1996
State/Local Invited Seminars/Lectures, Department of Chemistry and Biochemistry, University of Southern Mississippi1996
National and International Invited Seminars/Lectures, Department of Biophysics, Medical College of Wisconsin

Professional Membership and Service

Biochemistry, Reviewer, Ad Hoc Reviewer
Biochimica et Biophysica Acta Bioenergetics, Editorial Review Board Member
Biochimica et Biophysica Acta Bioenergetics, Reviewer, Ad Hoc Reviewer
Journal of Biological Chemistry, Reviewer, Ad Hoc Reviewer
Microbiology, Reviewer, Ad Hoc Reviewer
National Institutes of Health, Reviewer, Ad Hoc Reviewer
National Science Foundation, Reviewer, Ad Hoc Reviewer
The Wellcome Trust (United Kingdom), Reviewer, Ad Hoc Reviewer
UMC Intramural Research Support Program, Reviewer, Ad Hoc Reviewer
United States Department of Energy, Division of Energy Biosciences, Reviewer, Ad Hoc Reviewer
• Natural Sciences and Engineering Research Council of Canada, Reviewer, Ad Hoc Reviewer

Committee Service

Centrifuge & Ultralow Freezer, Coordinator
Medical Center - Institutional Biosafety
Preliminary Examination Committee, Chair
School Of Graduate Studies - Curriculum Committee
School Of Medicine - Curriculum

Teaching Experience

CMB 610, Medicine, Course, Lecturer, Medical BiochemistryFall Every
CMB 710, Graduate, Course, Lecturer, Graduate Medical BiochemistryFall Every
CMB 704, Graduate, Course, Lecturer, Fundamental BiochemistryFall Every
CMB 604, Dentistry, Course, Lecturer, Dental BiochemistryFall Every
CMB 711, Graduate, Course, Course Director, Mechanisms of Enzyme ActionSpring Even years
CMB 740, Graduate, Course, Lecturer, Biochemical MethodsSpring Every

Directed Student Learning

Dissertation Committee Chair, Advised: Ngoc Hoang2014 - 2019
Dissertation Committee Chair, Advised: Kristin Shirey2011 - 2016
Dissertation Committee Chair, Lipids and the D pathway of cytochrome c oxidase
Advised: Lakshman Varanasi
2004 - 2011
Dissertation Committee Chair, Copper chaperones in cytochrome oxidase assembly
Advised: Audie Thompson
2004 - 2011
Doctoral Advisory Committee Chair, Advised: Melyssa Bratton2000 - 2000
Doctoral Advisory Committee Member, Advised: Leslie Walker
Doctoral Advisory Committee Member, Advised: Nafez Tarboush
Doctoral Advisory Committee Member, Advised: Moonsung Choi
Doctoral Advisory Committee Member, Advised: Sooim Shin
Doctoral Advisory Committee Member, Advised: Patrizia Lemma-Gray
Doctoral Advisory Committee Member, Advised: Tingfeng Li
Doctoral Advisory Committee Member, Advised: John Ma
Doctoral Advisory Committee Member, Advised: Xianghui Li
Doctoral Advisory Committee Member, Advised: James McGeehee
Doctoral Advisory Committee Member, Advised: Yongting Wang
Doctoral Advisory Committee Member, Advised: Babette LaMarca
Doctoral Advisory Committee Member, Advised: Dapeng Sun
Doctoral Advisory Committee Member, Advised: Wenming Zhu
Doctoral Advisory Committee Member, Advised: Zhenyu Zhu
Doctoral Advisory Committee Member, Advised: Reid Bishop
Master's Thesis Committee Chair, Advised: J. Oca Cossio
Doctoral Advisory Committee Member, Advised: Liliana Falzon

Fellows supervised

Research Advisor, 1 fellows supervised01/2020 - Present
Research Advisor, 1 fellows supervised01/1998 - 01/2003