David T. Brown, PhD
Professor
Department:
SOM-Cell and Molecular Biology
Email:
dbrown@umc.edu
Work Phone:
(601) 984-1849
Education
| University of Mississippi Medical Center, PhD, Biochemistry | 1988 |
| Florida State University, MS, Genetics | 1981 |
| Florida State University, BS, Biology | 1976 |
Current Positions
| Professor, School of Medicine, Department of Biochemistry | 2004 - Present |
| The University of Mississippi Medical Center Jackson, Mississippi 39216 | |
Academic Appointments or Other Previous Appointments
| Associate Professor, The University of Mississippi Medical Center, School of Medicine, Department of Biochemistry | 2000 - 2004 |
| Assistant Professor, The University of Mississippi Medical Center, School of Medicine, Department of Biochemistry | 1993 - 2000 |
| Instructor, The University of Mississippi Medical Center, School of Medicine, Department of Biochemistry | 1991 - 1993 |
Publications
Journal Article | |
| Brown DT, Wellman SE, Sittman DB. Changes in the levels of three different classes of histone mRNA during murine erythroleukemia cell differentiation Mol. Cell. Biol. 1985 5:2879-2886 | |
| Catez F, Brown DT, Misteli T, Bustin M Competition between histone H1 and HMGN proteins for chromatin binding site EMBO Reports 2002; 3:760-766. | |
| Gunjan A, Sittman DB, Brown DB Core histone acetylation is regulated by linker histone stoichiometry in vivo J. Biol. Chem. 2001; 276:3635-3640 | |
| Brown DT, Alexander BT, Sittman DB Differential effect of H1 variant overexpression on cell cycle progression and gene expression Nucleic Acids Res. 1996; 24:486-493 | |
| Brown DT, Gunjan A, Alexander BT, Sittman DB Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain Nucleic Acids Res. 1997; 25:5003-5009 | |
| Becker M, Becker A, Miyara F, Han Z, Kihara M, Brown DT, Hager GL, Latham K, Adashi EY, Misteli T Differential in vivo binding dynamics of somatic and oocyte-specific linker histones in oocytes and during ES cell nuclear transfer Mol. Biol. Cell. 2005; 16:3887-3895 | |
| Stasevich, TJ, Mueller F, Brown DT, McNally JG Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis EMBO J. 2010; 29:1225-1234 | |
| Kumar A, Brown DT, Leno GH DNA intercalators differentially affect chromatin structure and DNA replication in Xenopus egg extract Anti-Cancer Drugs 2004; 15:633-639 | |
| Misteli T, Gunjan, A, Hock R, Bustin M, Brown DT Dynamic binding of histone H1 to chromatin in living cells Nature. 2000; 408:877-881 | |
| Gunjan A, Alexander BT, Sittman DB, Brown DT Effects of H1 histone variant over-expression on chromatin structure J. Biol. Chem. 1999; 274:37950-37956 | |
| Yellajoshyula D, Brown DT Global modulation of chromatin dynamics mediated by dephosphorylation of linker histone H1 is necessary for erythroid differentiation Proc. Natl. Acad. Sci. (USA) 2006; 103:18568-18573 | |
| Phair RD, Scaffidi P, Elbi C, Vecerova J, Dey A, Ozato K, Brown DT, Hager G, Bustin M, Misteli T Global nature of dynamic protein-chromatin interactions in vivo: three-deminsional scanning and dynamic interaction networks of chromatin proteins Mol. Cell. Biol. 2004; 24:6393-6404 | |
| Brown DT, Yang Y.-S., Sittman DB. Histone gene switching in murine erythroleukemia cells is differentiation specific and occurs without loss of cell cycle regulation Mol. Cell. Biol. 1988 8:4406-4415 | |
| Brown DT Histone H1 and the dynamic regulation of chromatin function Biochemistry Cell Biol. 2003; 81:221-227 | |
| Lu ZH, Sittman DB, Brown DT, Munshi R, Leno GH Histone H1 modulates DNA replication through multiple pathways in Xenopus egg extract J. Cell. Sci. 1997; 110:2745-2758 | |
| De S, Brown DT, Lu Z.H, Leno GH, Wellman SE, Sittman DB Histone H1 variants differentially inhibit DNA replication through an affinity for chromatin mediated by their carboxyl-terminal domains Gene, 2002; 292:173-181. | |
| Brown DT Histone variants: are they functionally heterogeneous? Genome Biology 2001; 2:6.1-6.6 | |
| Meshorer E, Yellajoshyula D, Goerge E, Scambler PJ, Brown DT, Misteli T Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells. Dev.Cell 2006; 10:105-116 | |
| Brown DT, Sittman DB Identification through overexpression and tagging of the variant type of the mouse H1e and H1c genes J. Biol. Chem. 1993; 268:713-718 | |
| Yang Y-S, Brown DT, Wellman SE, Sittman DB Isolation and characterization of a mouse fully replication-dependent H1 gene within a cluster of core histone genes J. Biol. Chem. 1987 262:17118-17125 | |
| Dong Y, Sirotkin AM, Yang Y.-S, Brown DT, Sittman DB, Skoultchi AI Isolation and characteriza¬tion of two replication-dependent mouse H1 histone genes Nucleic Acids Res.1994; 22:1421-1428 | |
| Brown DT, Izard T, Misteli T Mapping the interaction surface of linker histone H1(0) with the nucleosome of native chromatin in vivo Nat.Struct.Mol.Biol. 2006; 13:250-255 | |
| George EM, Izard T, Anderson SD, Brown DT Nucleosome interaction surface of linker histone H1c is distinct from that of H10 J. Biol. Chem. 2010; 285:20891-20896 | |
| Gunjan A, Brown DT Overproduction of histone H1 variants in vivo increases basal and hormone-induced activity of the mouse mammary tumor virus promoter Nucleic Acids Res. 1999; 27:3355-3363 | |
| Brunden KR, Brown DT. P0 mRNA expression in cultures of Schwann cells and neurons that lack basal lamina and myelin J. Neurosci. 1990 27:159-168 | |
| George EM, Brown DT Prothymosin α is a component of a linker histone chaperone FEBS Letters 2010; 584:2811-2836 | |
| Brown DT, Morris GF, Chodchoy N, Soprecher C, Marzluff WF Structure of the sea urchin U1 repeat Nucleic Acids Res. 1985 13:537-556 | |
| Chinthalapudi K, Rangarajan ES, Brown DT, Izard T, Differential lipid binding of vinculin isoforms promotes quasi-equivalent dimerization. Proceedings of the National Academy of Sciences of the United States of America, 2016 Aug 23;113(34):9539-44 | 2016 |
| Flanagan TW, Files JK, Casano KR, George EM, Brown DT, Photobleaching studies reveal that a single amino acid polymorphism is responsible for the differential binding affinities of linker histone subtypes H1.1 and H1.5. Biology open, 2016 Feb 24;5(3):372-80 | 2016 |
| Izard T, Brown DT, Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites. The Journal of biological chemistry, 2016 Feb 5;291(6):2548-55 | 2016 |
| Flanagan TW, Brown DT, Molecular dynamics of histone H1. Biochimica et biophysica acta, 2016 Mar;1859(3):468-75 | 2016 |
| Chinthalapudi K, Rangarajan ES, Patil DN, George EM, Brown DT, Izard T, Lipid binding promotes oligomerization and focal adhesion activity of vinculin. The Journal of cell biology, 2014 Dec 8;207(5):643-56 | 2014 |
| Jørgensen S, Dyhring T, Brown DT, Strøbæk D, Christophersen P, Demnitz J, A high-throughput screening campaign for detection of ca(2+)-activated k(+) channel activators and inhibitors using a fluorometric imaging plate reader-based tl(+)-influx assay. Assay and drug development technologies, 2013 Apr;11(3):163-72 | 2013 |
Editorial | |
| Brown DT, Izard T, Vinculin-cell membrane interactions. Oncotarget, 2015 Oct 27;6(33):34043-4 | 2015 |
Presentations
| State/Local Invited Seminars/Lectures, Fluorescent recovery after photobleaching (FRAP): amethod to study protein-nucleic acid binding in vivo, Inaugural Mississippi Biophysical Consortium, University of Mississippi, Oxford | 05/2008 |
| National and International Invited Seminars/Lectures, Role of H1 in modulating chromatin structure during the differentiation of mammalian cells, Department of Biomedical Sciences, Florida State College of Medicine | 09/2007 |
| Scientific Presentations, H1 - who needs it anyway, Department of Biochemistry, The University of Mississippi Medical Center | 2007 |
| National and International Invited Seminars/Lectures, Mapping the interaction surface of the linker histone H1 with the nucleosomes of native chromatin in vivo, 27th Annual Aswilomar Chromatin & Chromosomes Conference | 2005 |
| Scientific Presentations, Mapping H1 binding interactions by fluroescent recovery after photobleaching, Department of Biochemistry, The University of Mississippi Medical Center | 2003 |
| Scientific Presentations, Analysis of histone H1 binding to nucleosomes in living cells, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota | 2002 |
| Scientific Presentations, Dynamic interaction of histone H1 with chromatin in living cells, Department of Structural Biology, St. Jude Children's Hospital | 2002 |
| National and International Invited Seminars/Lectures, Dynamics of histone H1 binding to chromatin in living cells | 2002 |
| Scientific Presentations, Dynamic interation of histone H1 with chromatin in living cells, Stritch School of Medicine, Loyola University Chicago | 2001 |
| Scientific Presentations, Dynamics of histone H1 binding to chromatin in living cells, Department of Biochemistry, The University of Mississippi Medical Center | 2001 |
| Scientific Presentations, Ethical considerations of the cloning of mammalian organisms, Department of Business Administration, Belhaven College, MS | 1999 |
| Scientific Presentations, Linker histone and chromatin structure and function: in vivo studies, National Institutes of Health, National Cancer Institute, Bethesda, MD | 1999 |
| Scientific Presentations, Over-expression of linker histone variants reduces the rate of spontaneous and benzo(a)pyrene-induced transformation of murine BASL/c 3T3 cells, American Cancer Society Site Visit, The University of Mississippi Medical Center | 1999 |
| Scientific Presentations, Role of H1 in transcriptional regulation, Department of Physiology, The University of Mississippi Medical Center | 1999 |
| Scientific Presentations, Effects of H1 Variant Overexpression on Chromatin Structure, Transcription of the MMTV Promoter, and Cell Cycle Progression, Department of Biochemistry, The University of Mississippi Medical Center | 1998 |
| Scientific Presentations, Role of H1 Variants in Cell Cycle Progression and Oncogenesis, American Cancer Society Site Visit, University of Mississippi Medical Center | 1997 |
| Scientific Presentations, Effects of H1 Variant Overexpression on Transcription and Cell Cycle Progression, Department of Biochemistry, The University of Mississippi Medical Center | 1995 |
| Scientific Presentations, Methods for Introduction of DNA into Mammalian Cells and Targeted Gene Inactivation, Department of Physiology, The University of Mississippi Medical Center | 1995 |
| Scientific Presentations, Effects of H1 Variant Overexpression on Transcription and Cell Cycle Progression, Department of Biochemistry, Mississippi State University | 1994 |
| Scientific Presentations, Effects of H1 Variant Overexpression on Transcription and Cell Cycle Progression, Department of Pharmacology, The University of Mississippi Medical Center | 1994 |
| Scientific Presentations, Functional Significance of H1 Variant Heterogeneity, Department of Physiology, The University of Mississippi Medical Center | 1994 |
| Scientific Presentations, Transient Inhibition of DNA Replication in H1 variant-overexpressing cell lines is associated with a high H1/nucleosome ratio, Department of Biochemistry, The University of Mississippi Medical Center | 1993 |
| Scientific Presentations, Structure of mouse and sea urchin small nuclear RNA genes, Department of Chemistry, Florida State University | 1983 |
| Scientific Presentations, Basics of Biomedical Research and Ethical Responsibilities of Data Collection, Analysis and Reporting, Department of Psychiatry, University of Mississippi Medical Center |
Honors, Awards, and Recognition
| Bronz Award for Excellence in Research | 2003 |
| Thomas M. (Peter) Blake Academy of Teaching Excellence | 2003 |
| Honor Society of Phi Kappa Phi | 1987 |
| Robert A Mahaffey Graduate Student Research Award, The University of Mississippi Medical Center | 1986 |
| University Fellowship, Florida State University | 1977 |
| Undergraduate Research Award, Florida State University | 1976 |
Committee Service
| School Of Medicine - Admissions, Member | 2004 - 2008 |
| School Of Medicine - Curriculum, Member | 2002 - 2010 |
Teaching Experience
| 610, Medicine, Course, Lecturer, Medical Biochemistry | Fall 2008 |
| 744, Graduate, Course, Lecturer, Molecular Cell Biology | 2008 |
| 610, Medicine, Course, Lecturer, Medical Biochemistry | Fall 2007 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 2007 |
| 610, Medicine, Course, Lecturer, Medical Biochemistry | Fall 2006 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 2006 |
| 744, Graduate, Course, Lecturer, Molecular Cell Biology | 2006 |
| 610, Medicine, Course, Course Director, Medical Biochemistry | Fall 2005 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 2005 |
| 610, Medicine, Course, Course Director, Medical Biochemistry | Fall 2004 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 2004 |
| 744, Graduate, Course, Lecturer, Molecular Cell Biology | 2004 |
| 610, Medicine, Course, Lecturer, Medical Biochemistry | Fall 2003 |
| 610, Medicine, Course, Course Director, Medical Biochemistry | Fall 2003 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 2003 |
| 742, Graduate, Course, Lecturer, Molecular Biology Methods | 2003 |
| 604, Dentistry, Lecture, Lecturer, Dental Biochemistry | Fall 2002 |
| 610, Medicine, Course, Lecturer, Medical Biochemistry | Fall 2002 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 2002 |
| 744, Graduate, Course, Lecturer, Molecular Cell biology | 2002 |
| 610, Medicine, Course, Lecturer, Medical Biochemistry | Fall 2001 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 2001 |
| 742, Medicine, Course, Lecturer, Molecular Biology Methods | 2001 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 2000 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 2000 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1999 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 1999 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1998 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 1998 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1997 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 1997 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1996 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 1996 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1995 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 1995 |
| 720, Medicine, Course, Lecturer, Molecular Genetics | 1995 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1994 |
| 710, Graduate, Course, Course Director, Graduate Biochemistry | 1994 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1993 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 1993 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | Fall 1992 |
| 740, Medicine, Course, Lecturer, Biochemical Methods | 1992 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 1992 |
| 710, Graduate, Course, Lecturer, Graduate Biochemistry | 1991 |
| 610, Medicine, Lecture, Lecturer, Medical Biochemistry | 1991 |
| 604, Dentistry, Lecture, Lecturer, Dental Biochemistry | Fall 1990 |
Directed Student Learning
| Dissertation Committee Chair, Undetermined Advised: Payal Vyas | 2003 - Present |
| Dissertation Committee Chair, Advised: Akash Gunjan | |
| Dissertation Committee Chair, Advised: Asmita Kumar | |
| Dissertation Committee Chair, Advised: Dhananjay Yellajoshula | |
| Dissertation Committee Chair, Determinants of linker histone binding and dynamics Advised: Eric George | |
| Dissertation Committee Chair, Advised: Rajesh Savkur | |
| Dissertation Committee Member, Advised: Ashley Herring | |
| Dissertation Committee Member, Advised: Barbara Alexander | |
| Dissertation Committee Member, Advised: Gene Bidwell | |
| Dissertation Committee Member, Advised: Holland Alday | |
| Dissertation Committee Member, Advised: Naved Alam | |
| Dissertation Committee Member, Advised: Nian Huang | |
| Dissertation Committee Member, Advised: Oca Cassio | |
| Dissertation Committee Member, Advised: Rajan Munshi | |
| Dissertation Committee Member, Advised: Shama Moktan | |
| Dissertation Committee Member, Advised: Sheetal Bhan | |
| Dissertation Committee Member, Advised: Sidhartha De | |
| Dissertation Committee Member, Advised: Valeria Cuchiarelli | |
| Dissertation Committee Member, Advised: Zhi Hong Lu |